Article
Selection and characterization of beta-lactam-beta-lactamase inactivator-resistant mutants following PCR mutagenesis of the TEM-1 beta-lactamase gene.
Antimicrobial agents and chemotherapy - 1 Jul 1998
Vakulenko S B, Geryk B, Kotra L P, Mobashery S, Lerner S A
Abstract excerpt
Mechanism-based inactivators of beta-lactamases are used to overcome the resistance of clinical pathogens to beta-lactam antibiotics. This strategy can itself be overcome by mutations of the beta-lactamase that compromise the effectiveness of their inactivation. We used PCR mutagenesis of the TEM...
Topics
- Ampicillin
- Anti-Bacterial Agents
- Clavulanic Acid
- Drug Resistance, Multiple
- Enzyme Inhibitors
- Escherichia coli
- Microbial Sensitivity Tests
- Models, Molecular
- Mutagenesis
- Penicillins
- Phenotype
- Polymerase Chain Reaction
- beta-Lactam Resistance
- beta-Lactamase Inhibitors
- beta-Lactamases