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Rapid, High Level Protein Production Using DNA-based Semliki Forest Virus Vectors

1 Jul 1998

Abstract excerpt

Semliki Forest virus (SFV) vectors can be produced faster, and have a wider host range, than baculovirus vectors. However, the original SFV system requires in vitro manipulation of RNA. We have generated a system that is wholly DNA-based. Both the replicon vector, encoding SFV polymerase and the protein of interest, and the helper vector, encoding viral structural proteins, were modified so that expression was...

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PMID: 9660762
DOI: 10.1074/jbc.273.29.18060

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