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Retinoblastoma Protein Purification and Transduction of Retina and Retinoblastoma Cells Using Improved Alphavirus Vectors

23 Aug 2004

Abstract excerpt

PURPOSE: To develop and use improved Semliki Forest vectors (SFVs) for functional and structural analyses of the retinoblastoma protein (RB) in developing retina and retinoblastoma cells. METHODS: Virus was harvested from cells transfected with replicon and helper plasmids. Combinations of producer and target cells were tested for optimal virus production and protein expression. The replicon was improved by...

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PMID: 15326157
DOI: 10.1167/iovs.04-0140

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