Article
Enzyme-complemented activatorsorbent assay (ECASA): genetic engineering for enzyme-linked immunosorbent assay-type mercuric ion detection.
Analytical biochemistry - 1 Jul 1998
Klein J, Mattes R
Abstract excerpt
The sensor component of bacterial mercury resistance systems is the metalloregulatory protein MerR, which has nanomolar sensitivity and high selectivity for Hg(II). A fusion protein of MerR and the alpha-peptide part of beta-galactosidase (LacZalpha) was constructed by fusing the relevant genes....
Topics
- Bacterial Proteins
- Cloning, Molecular
- DNA-Binding Proteins
- Drug Resistance, Microbial
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Genetic Complementation Test
- Genetic Engineering
- Genotype
- Mercury
- Plasmids
- Recombinant Fusion Proteins
- Restriction Mapping
- Sensitivity and Specificity
- beta-Galactosidase