Article
Construction of a synthetic gene for the metalloregulatory protein MerR and analysis of regionally mutated proteins for transcriptional regulation.
Biochemistry - 12 Apr 1994
Comess K M, Shewchuk L M, Ivanetich K, Walsh C T
Abstract excerpt
The transcriptional control protein MerR is a metalloregulatory switch, activating transcription of a mercury resistance operon in the presence of mercuric ions and repressing transcription in their absence. We report here the construction and utilization of a synthetic merR gene and a single-cop...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA-Binding Proteins
- Escherichia coli
- Gene Expression Regulation
- Genes, Synthetic
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Phenanthrolines
- Recombinant Proteins
- Transcription, Genetic