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Article

Construction of a synthetic gene for the metalloregulatory protein MerR and analysis of regionally mutated proteins for transcriptional regulation.

Biochemistry - 12 Apr 1994

Comess K M, Shewchuk L M, Ivanetich K, Walsh C T

Abstract excerpt

The transcriptional control protein MerR is a metalloregulatory switch, activating transcription of a mercury resistance operon in the presence of mercuric ions and repressing transcription in their absence. We report here the construction and utilization of a synthetic merR gene and a single-copy merT'-lacZ fusion reporter for mutagenic analysis of the MerR protein's function. Site-directed mutagenesis of...

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PMID: 8155633
DOI: 10.1021/bi00180a010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Construction of a synthetic gene for the metalloregulatory protein MerR and analysis of regionally mutated proteins for transcriptional regulation.PMID 8155633Read ↗

Cd(II)-responsive and constitutive mutants implicate a novel domain in MerR.Semantic proximity Read ↗

Construction and characterization of a mercury-independent MerR activator (MerRAC): transcriptional activation in the absence of Hg(II) is accompanied by DNA distortion.Semantic proximity Read ↗

The MerR metalloregulatory protein binds mercuric ion as a tricoordinate, metal-bridged dimer.Semantic proximity Read ↗

Selection and characterization of mercury-independent activation mutants of the Tn501 transcriptional regulator, MerR.Semantic proximity Read ↗

Purification and functional characterization of MerD. A coregulator of the mercury resistance operon in gram-negative bacteria.Semantic proximity Read ↗

Site-specific insertion and deletion mutants in the mer promoter-operator region of Tn501; the nineteen base-pair spacer is essential for normal induction of the promoter by MerR.Semantic proximity Read ↗

Mutations in the alpha and sigma-70 subunits of RNA polymerase affect expression of the mer operon.Semantic proximity Read ↗

In vivo DNA-protein interactions at the divergent mercury resistance (mer) promoters. II. Repressor/activator (MerR)-RNA polymerase interaction with merOP mutants.Semantic proximity Read ↗

Overexpression of MerT, the mercuric ion transport protein of transposon Tn501, and genetic selection of mercury hypersensitivity mutations.Semantic proximity Read ↗

The role of cysteine residues in the transport of mercuric ions by the Tn501 MerT and MerP mercury-resistance proteins.Semantic proximity Read ↗

Identification of a putative metal binding site in a new family of metalloregulatory proteins.Semantic proximity Read ↗

NmlR of Neisseria gonorrhoeae: a novel redox responsive transcription factor from the MerR family.Semantic proximity Read ↗

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