Article
LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion.
Cell - 26 Jun 1998
Xu Z, Sato K, Wickner W
Abstract excerpt
Vacuole fusion requires Sec18p (NSF), Sec17p (alpha-SNAP), Ypt7p (GTP binding protein), Vam3p (t-SNARE), Nyv1p (v-SNARE), and LMA1 (low Mr activity 1, a heterodimer of thioredoxin and I(B)2). LMA1 requires Sec18p for saturable, high-affinity binding to vacuoles, and Sec18p "priming" ATPase requir...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Sequence
- Enzyme Inhibitors
- Fungal Proteins
- Glycoproteins
- Hydrolysis
- Marine Toxins
- Membrane Fusion
- Membrane Proteins
- Microcystins
- Molecular Sequence Data
- Mutation
- Peptides, Cyclic
- Phosphoprotein Phosphatases
- Phosphoproteins
- Protein Binding
- Qa-SNARE Proteins