Chemical denaturation: potential impact of undetected intermediates in the free energy of unfolding and m-values obtained from a two-state assumption.

doi:10.1016/S0006-3495(98)77537-X

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Article

Chemical denaturation: potential impact of undetected intermediates in the free energy of unfolding and m-values obtained from a two-state assumption.

Biophysical journal - 1 Jul 1998

Soulages J L

Abstract excerpt

The chemical unfolding transition of a protein was simulated, including the presence of an intermediate (I) in equilibrium with the native (N) and unfolded (U) states. The calculations included free energies of unfolding, DeltaGuw, in the range of 1.4 kcal/mol to 10 kcal/mol and three different g...

Topics

Biophysical Phenomena
Biophysics
Circular Dichroism
Models, Chemical
Mutation
Protein Denaturation
Protein Folding
Protein Structure, Secondary
Proteins
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Thermodynamics

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