Article
A distinct contribution of the delta subunit to acetylcholine receptor channel activation revealed by mutations of the M2 segment.
Biophysical journal - 1 Jul 1998
Chen J, Auerbach A
Abstract excerpt
Acetylcholine receptor (AChR) channels with proline (P) mutations in the putative pore-forming domain (at the 12' position of the M2 segment) were examined at the single-channel level. For all subunits (alpha, beta, epsilon, and delta), a 12'P mutation increased the open channel lifetime >5-fold....
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Biophysical Phenomena
- Biophysics
- Cell Line
- Humans
- Ion Channel Gating
- Kinetics
- Mice
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Receptors, Cholinergic
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Transfection