Quantitative restriction fragment length polymorphism: a procedure for quantitation of diphtheria toxin gene CRM197 allele.

doi:10.1006/abio.1998.2682

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Article

Quantitative restriction fragment length polymorphism: a procedure for quantitation of diphtheria toxin gene CRM197 allele.

Analytical biochemistry - 15 Jun 1998

Pushnova E A, Zhu Y S

Abstract excerpt

Here we present an assay for quantitation of a particular gene allele in DNA mixtures by means of restriction fragment length polymorphism (RFLP) in combination with polymerase chain reaction (PCR). We applied the quantitative RFLP principle for estimation of the relative amount of diphtheria tox...

Topics

Alleles
Bacterial Proteins
Corynebacterium diphtheriae
DNA, Bacterial
Diphtheria Toxin
Electrophoresis, Polyacrylamide Gel
Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reproducibility of Results

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