Article
CD34+, kit+, rhodamine123(low) phenotype identifies a marrow cell population highly enriched for human hematopoietic stem cells.
Leukemia - 1 Jun 1998
Ratajczak M Z, Pletcher C H, Marlicz W, Machalinski B, Moore J, Wasik M, Ratajczak J, Gewirtz A M
Abstract excerpt
We hypothesized that human hematopoietic cells displaying a CD34+, kit-, rhodamine123(low) phenotype would be highly enriched for cells with stem-like properties. To test this hypothesis, we employed fluorescence activated cell sorting (FACS) to isolate cells with this phenotype from normal light...
Topics
- Animals
- Antigens, CD34
- Bone Marrow Cells
- Cell Separation
- Cells, Cultured
- Hematopoietic Stem Cell Transplantation
- Hematopoietic Stem Cells
- Humans
- Mice
- Mice, SCID
- Phenotype
- Proto-Oncogene Proteins c-kit
- Rhodamine 123
- Rhodamines
