Article
CD34+, kit+, rhodamine123(low) phenotype identifies a marrow cell population highly enriched for human hematopoietic stem cells.
Leukemia - 1 Jun 1998
Ratajczak M Z, Pletcher C H, Marlicz W, Machalinski B, Moore J, Wasik M, Ratajczak J, Gewirtz A M
Abstract excerpt
We hypothesized that human hematopoietic cells displaying a CD34+, kit-, rhodamine123(low) phenotype would be highly enriched for cells with stem-like properties. To test this hypothesis, we employed fluorescence activated cell sorting (FACS) to isolate cells with this phenotype from normal light density marrow mononuclear cells (MNC). CD34+, kit+, rhodamine123(low) cells comprised from 0.05-0.01% of the total...
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