Article
New paramagnetic species formed at the expense of the transient tyrosyl radical in mutant protein R2 F208Y of Escherichia coli ribonucleotide reductase.
Biochemical and biophysical research communications - 29 May 1998
Liu A, Sahlin M, Pötsch S, Sjöberg B M, Gräslund A
Abstract excerpt
The highly conserved residue F208 in protein R2 of E. coli ribonucleotide reductase is close to the binuclear iron center, and found to be involved in stabilizing the tyrosyl radical Y122. in wild type R2. Upon the reconstitution reaction of the mutant R2 F208Y with ferrous iron and molecular oxygen, we observed a new EPR singlet signal (g = 2.003) formed concomitantly with decay of the transient tyrosyl radical...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.