Article
The development and evaluation of a probe hybridization method for subtyping HIV type 1 infection in Uganda.
AIDS research and human retroviruses - 20 May 1998
Luo C C, Downing R G, Dela Torre N, Baggs J, Hu D J, Respess R A, Candal D, Carr L, George J R, Dondero T J, Biryahwaho B, Rayfield M A
Abstract excerpt
We developed a method for large-scale screening of HIV-1 genotypic variation based on DNA probe hybridization. Nested PCR amplifications were performed to generate fragments in the env C2-V3 region and also in the gp41 region, which encompasses the immunodominant domain. The proviral DNA sequences were derived from 68 samples and phylogenetically analyzed. For comparison, the C2-V3 fragment was used in DNA probe...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
