Article
Genotype-specific RNA probes for direct identification of wild polioviruses by blot hybridization.
Journal of clinical microbiology - 1 Nov 1997
De L, Yang C F, Da Silva E, Boshell J, Cáceres P, Gómez J R, Pallansch M, Kew O
Abstract excerpt
We have developed RNA probes for the direct identification of wild poliovirus isolates by blot hybridization. The probes are complementary to sequences of the first 30 to 32 codons of VP1, which evolve more extensively (approximately 1.5-fold) than the rest of VP1. To illustrate our general approach, we describe the design of probes specific to each of four major genotypes recently endemic (1981 to 1991) to the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.