Article
Sequence analysis of the V3 loop regions of the env genes of Ugandan human immunodeficiency proviruses.
AIDS research and human retroviruses - 1 Jul 1991
Oram J D, Downing R G, Roff M, Serwankambo N, Clegg J C, Featherstone A S, Booth J C
Abstract excerpt
Ugandan strains of human immunodeficiency virus type 1 (HIV-1) were isolated by cocultivation of peripheral blood lymphocytes from infected individuals with cord blood lymphocytes. Sequences from the V3 region of the env gene were amplified by the polymerase chain reaction (PCR) from chromosomal DNA obtained from low passage virus cultures. The PCR products from 13 Ugandan isolates were cloned into a phagemid...
Topics
- Acquired Immunodeficiency Syndrome
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Viral
- Genes, env
- Genetic Variation
- HIV Envelope Protein gp120
- HIV-1
- Humans
- Molecular Sequence Data