Article
Direct demonstration of Ca2+ binding defects in sarco-endoplasmic reticulum Ca2+ ATPase mutants overexpressed in COS-1 cells transfected with adenovirus vectors.
The Journal of biological chemistry - 12 Jun 1998
Strock C, Cavagna M, Peiffer W E, Sumbilla C, Lewis D, Inesi G
Abstract excerpt
Single mutations of specific amino acids within the membrane-bound region of the sarco-endoplasmic reticulum Ca2+ (SERCA)-1 ATPase interfere with Ca2+ inhibition of ATPase phosphorylation by Pi (1), suggesting that these residues may be involved in complexation of two Ca2+ that are known to bind...
Topics
- Adenosine Triphosphate
- Adenoviridae
- Animals
- COS Cells
- Calcium
- Calcium-Binding Proteins
- Calcium-Transporting ATPases
- Endoplasmic Reticulum
- Gene Transfer Techniques
- Genetic Vectors
- Hydrogen-Ion Concentration
- Microscopy, Fluorescence
- Microsomes
- Mutation