Article
Mutation of glutamate 309 to glutamine alters one Ca(2+)-binding site in the Ca(2+)-ATPase of sarcoplasmic reticulum expressed in Sf9 cells.
The Journal of biological chemistry - 25 Jul 1993
Skerjanc I S, Toyofuku T, Richardson C, MacLennan D H
Abstract excerpt
Sf9 cells infected with a baculovirus vector containing SERCA1 cDNA expressed immunoreactive rabbit fast-twitch muscle Ca(2+)-ATPase at levels up to 3 mg/liter. The microsomal fraction isolated from infected Sf9 cells catalyzed Ca2+ transport at rates 6-fold above control values. To obtain direct...
Topics
- Animals
- Baculoviridae
- Base Sequence
- Binding Sites
- Calcium
- Calcium-Transporting ATPases
- Cells, Cultured
- Cloning, Molecular
- Glutamates
- Glutamic Acid
- Glutamine
- Molecular Sequence Data
- Moths
- Mutation
- Phosphorylation
- Rabbits
- Sarcoplasmic Reticulum