Article
Mutation of conserved residues in transmembrane domains 4,6 and 8 causes loss of Ca2+ transport by the plasma membrane Ca2+ pump.
Biochemistry - 12 Mar 1996
Guerini D, Foletti D, Vellani F, Carafoli E
Abstract excerpt
Mutants of the plasma membrane Ca2+ pump (PMCA), in which amino acids in transmembrane domains (TM) 4, 6, and 8 had been replaced, have been expressed in COS-7 cells. They were analyzed functionally by measuring the uptake of Ca2+ in microsomal preparations and by following the formation of the p...
Topics
- Adenosine Triphosphate
- Animals
- Base Sequence
- Biological Transport
- Calcium
- Calcium-Transporting ATPases
- Cation Transport Proteins
- Cell Compartmentation
- Cell Membrane
- Cells, Cultured
- Conserved Sequence
- Immunohistochemistry
- Microsomes
- Molecular Sequence Data
- Mutation
- Phosphates
- Phosphorylation
- Plasma Membrane Calcium-Transporting ATPases