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Article

Detection of a tryptophan radical as an intermediate species in the reaction of horseradish peroxidase mutant (Phe-221 --> Trp) and hydrogen peroxide.

The Journal of biological chemistry - 12 Jun 1998

Morimoto A, Tanaka M, Takahashi S, Ishimori K, Hori H, Morishima I

Abstract excerpt

The crucial reaction intermediate in the reaction of peroxidase with hydrogen peroxide (H2O2), compound I, contains a porphyrin pi-cation radical in horseradish peroxidase (HRP), which catalyzes oxidation of small organic and inorganic compounds, whereas cytochrome c peroxidase (CcP) has a radica...

Topics

Calcium
Cytochrome-c Peroxidase
Electron Spin Resonance Spectroscopy
Free Radicals
Horseradish Peroxidase
Hydrogen Peroxide
Kinetics
Magnetic Resonance Spectroscopy
Models, Molecular
Mutagenesis, Site-Directed
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Porphyrins

Recombinant Proteins

Spectrophotometry

Tryptophan

Source

Identifiers

PMID: 9614074
DOI: 10.1074/jbc.273.24.14753

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9614074-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Detection of a tryptophan radical as an intermediate species in the reaction of horseradish peroxidase mutant (Phe-221 --> Trp) and hydrogen peroxide.PMID 9614074Read ↗

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