Article
[The improvement of a direct DNA sequencing method by devising the primer design: detection of lipoprotein lipase gene aberrations].
Rinsho byori. The Japanese journal of clinical pathology - 1 Oct 1996
Mori A, Takagi A, Ikeda Y, Yamamoto A
Abstract excerpt
We developed an improved method of direct DNA sequencing which makes it possible to detect heterozygous mutations in an exon and the splicing consensus regions (acceptor and donor) in introns even in the cases where there is limited information available regarding the base sequences of the introns flanking the exon. Human lipoprotein lipase (LPL) gene was utilized to develop this method, since the reported intron...
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