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Article

Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore.

Nature biotechnology - 1 Oct 2000

Movileanu L, Howorka S, Braha O, Bayley H

Abstract excerpt

Here we describe a new type of biosensor element for detecting proteins in solution at nanomolar concentrations. We tethered a 3.4 kDa polyethylene glycol chain at a defined site within the lumen of the transmembrane protein pore formed by staphylococcal alpha-hemolysin. The free end of the polymer was covalently attached to a biotin molecule. On incorporation of the modified pore into a lipid bilayer, the...

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Identifiers

PMID: 11017049
DOI: 10.1038/80295

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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