Article
A DNA fragment containing the ADE2 gene from Schwanniomyces occidentalis can be maintained as an extrachromosomal element.
Gene - 15 Jan 1991
Klein R D, Favreau M A
Abstract excerpt
A 4.05-kb DNA fragment containing the ADE2 gene from Schwanniomyces occidentalis was cloned into the pUC19 vector. When an ade2 strain of Sc. occidentalis was transformed with this plasmid, pADE-2 was found to integrate into the host chromosome and was also present in a variety of extrachromosomal species. These extrachromosomal elements were present in multiple copies, varied in molecular mass and were composed...
Topics
- Blotting, Southern
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Phenotype
- Plasmids
- Restriction Mapping
- Saccharomycetales
- Transformation, Genetic