Article
Characterization of human leukocyte-HUVEC adhesion: Effect of cell preparation methods.
Journal of immunological methods - 1 Apr 2017
Marino Franca, Schembri Laura, Rasini Emanuela, Pinoli Monica, Scanzano Angela, Luini Alessandra, Congiu Terenzio, Cosentino Marco
Abstract excerpt
OBJECTIVE: Sample manipulation to obtain isolated granulocytes represents a key, and often necessary, step in the in vitro studies. We investigated by the means of flow cytometry and microscopic techniques (both optical microscopy [OM] and scanning electron microscopy [SEM]), the granulocyte-endothelium adhesion and the role of sample manipulation. METHODS: By means of a co-culture method, we have analysed the...
Topics
- CD18 Antigens
- Cell Adhesion
- Cell Adhesion Molecules
- Cell Separation
- Cell Shape
- Cells, Cultured
- Centrifugation, Density Gradient
- Coculture Techniques
- E-Selectin
- Flow Cytometry
- Human Umbilical Vein Endothelial Cells
- Humans
- Intercellular Adhesion Molecule-1