Article
Combined boyden-flow cytometry assay improves quantification and provides phenotypification of leukocyte chemotaxis.
PloS one - 1 Jan 2011
Gomez-Lopez Nardhy, Vadillo-Ortega Felipe, Estrada-Gutierrez Guadalupe
Abstract excerpt
Chemotaxis has been studied by classical methods that measure chemotactic and random motility responses in vitro, but these methods do not evaluate the total number and phenotype of migrating leukocytes simultaneously. Our objective was to develop and validate a novel assay, combined Boyden-flow cytometry chemotaxis assay (CBFCA), for simultaneous quantification and phenotypification of migrating leukocytes....
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