Article
The in vitro lymphocyte/endothelium binding assay. An improved method employing light microscopy.
Journal of immunological methods - 3 Jul 1990
Willführ K U, Hirschfeld U, Westermann J, Pabst R
Abstract excerpt
The in vitro lymphocyte binding assay (HEV assay) has proved to be a useful approach for examining the first step of lymphocyte migration, i.e., homing to organs containing high endothelial venules (HEVs). Since fluorescence-labelled standard lymphocytes are usually included in each assay to acco...
Topics
- Animals
- Antigens, Surface
- Cell Adhesion
- Cell Movement
- Endothelium, Vascular
- Fluorescein-5-isothiocyanate
- Fluoresceins
- Histological Techniques
- Immunoenzyme Techniques
- Immunologic Techniques
- In Vitro Techniques
- Lymphocytes
- Male
- Microscopy
- Phenotype
- Preservation, Biological
- Rats
- Rats, Inbred Lew