A ribosomal function is necessary for efficient splicing of the T4 phage thymidylate synthase intron in vivo.

doi:10.1101/gad.12.9.1327

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Article

A ribosomal function is necessary for efficient splicing of the T4 phage thymidylate synthase intron in vivo.

Genes & development - 1 May 1998

Semrad K, Schroeder R

Abstract excerpt

Splicing of the group I intron of the T4 thymidylate synthase (td) gene was uncoupled from translation by introducing stop codons in the upstream exon. This resulted in severe splicing deficiency in vivo. Overexpression of a UGA suppressor tRNA partially rescued splicing, suggesting that this in...

Topics

Bacteriophage T4
Base Sequence
Codon, Terminator
DNA Primers
Escherichia coli
Exons
Genes, Viral
Introns
Mutation
Protein Biosynthesis
RNA Splicing
RNA, Bacterial
RNA, Messenger
Ribosomal Proteins
Ribosomes
Thymidylate Synthase

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