Article
Fatty acid elongation in yeast--biochemical characteristics of the enzyme system and isolation of elongation-defective mutants.
European journal of biochemistry - 15 Mar 1998
Dittrich F, Zajonc D, Hühne K, Hoja U, Ekici A, Greiner E, Klein H, Hofmann J, Bessoule J J, Sperling P, Schweizer E
Abstract excerpt
Elongation of long-chain fatty acids was investigated in yeast mutants lacking endogenous de novo fatty acid synthesis. In this background, in vitro fatty acid elongation was dependent strictly on the substrates malonyl-CoA, NADPH and a medium-chain or long-chain acyl-CoA primer of 10 or more car...
Topics
- Acyl Coenzyme A
- Acyl-Carrier Protein S-Malonyltransferase
- Acyltransferases
- Cloning, Molecular
- Fatty Acids, Nonesterified
- Genes, Fungal
- Genetic Complementation Test
- Genotype
- Kinetics
- Saccharomyces cerevisiae
- Substrate Specificity