Article
Evaluation and mapping of the DNA binding and oligomerization domains of the IE2 regulatory protein of human cytomegalovirus using yeast one and two hybrid interaction assays.
Gene - 27 Mar 1998
Ahn J H, Chiou C J, Hayward G S
Abstract excerpt
The 86-kDa IE2 nuclear phosphoprotein encoded by the human cytomegalovirus (HCMV) major immediate-early (MIE) gene behaves as both a non-specific transactivator of viral and cellular gene expression and as a specific DNA-binding protein targeted to the cis-repression sequence (CRS) at the cap sit...
Topics
- Animals
- Chlorocebus aethiops
- DNA-Binding Proteins
- Dimerization
- Gene Expression Regulation, Viral
- Genes, Reporter
- Humans
- Immediate-Early Proteins
- Membrane Glycoproteins
- Mutation
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae Proteins
- Trans-Activators
- Transcription Factors
- Transfection
- Vero Cells
- Viral Envelope Proteins