An in vitro system for human cytomegalovirus immediate early 2 protein (IE2)-mediated site-dependent repression of transcription and direct binding of IE2 to the major immediate early promoter.

doi:10.1073/pnas.90.2.707

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Article

An in vitro system for human cytomegalovirus immediate early 2 protein (IE2)-mediated site-dependent repression of transcription and direct binding of IE2 to the major immediate early promoter.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jan 1993

Macias M P, Stinski M F

Abstract excerpt

In vivo, negative autoregulation of the strong major immediate early promoter (MIEP) of human cytomegalovirus requires the viral immediate early 2 protein (IE2) and a cis element located from position -13 through position -1 relative to the transcription start site. We have established an in vitr...

Topics

Base Sequence
Cell Nucleus
Cell-Free System
Cytomegalovirus
Gene Expression Regulation, Viral
HeLa Cells
Humans
Immediate-Early Proteins
Membrane Glycoproteins
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nuclear Proteins

Peptide Fragments

Promoter Regions, Genetic

Recombinant Fusion Proteins

Regulatory Sequences, Nucleic Acid

Trans-Activators

Transcription, Genetic

Source

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Identifiers

PMID: 8380646
DOI: 10.1073/pnas.90.2.707
PMCID: PMC45734

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8380646-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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