Article
pH dependence of the function of sodium ion extrusion systems in Escherichia coli.
Biochimica et biophysica acta - 25 Mar 1998
Sakuma T, Yamada N, Saito H, Kakegawa T, Kobayashi H
Abstract excerpt
Escherichia coli has three systems for sodium ion extrusion, NhaA, NhaB and ChaA. In this study, we examined the effect of pH on the function of these transporters using mutants having one of them, and found that (1) a mutant having NhaB excreted sodium ions at pH 7.5 but not at pH 8.5, (2) the efflux of sodium ions from mutant cells having ChaA was observed at both pH 7.5 and 8.5, but the activity was lower at...
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