Article
Cytochrome-c-binding site on cytochrome oxidase in Paracoccus denitrificans.
European journal of biochemistry - 15 Jan 1998
Witt H, Malatesta F, Nicoletti F, Brunori M, Ludwig B
Abstract excerpt
To monitor the docking site for cytochrome c on cytochrome oxidase from Paracoccus denitrificans, a series of site-directed mutants in acidic residues exposed on the three largest subunits was constructed, and the purified enzymes were assayed for their steady-state kinetic parameters, their ionic strength dependence, and their fast electron entry kinetics by stopped-flow measurements. Increasing the ionic...
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