Article
DNA bending and expression of the divergent nagE-B operons.
Nucleic acids research - 1 Mar 1998
Plumbridge J, Kolb A
Abstract excerpt
Repression of the divergent nagE - B operons requires NagC binding to two operators which overlap the nagE and nagB promoters, resulting in formation of a DNA loop. Binding of the cAMP/CAP activator to its site, adjacent to the nagE operator, stabilizes the DNA loop in vitro. The DNA of the nagE-B intergenic region is intrinsically bent, with the bend centred on the CAP site. We show that displacement of the CAP...
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