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Article

[Single-fiber polymerase chain reaction for detection of mutant mitochondrial DNA].

Nihon rinsho. Japanese journal of clinical medicine - 1 Dec 1997

Tokunaga M, Mita S, Uchino M

Abstract excerpt

Single-fiber PCR amplifies mitochondrial DNA (mtDNA) in single muscle fiber isolated from cross frozen section. The PCR products are digested with a restriction enzyme to distinguish mutant mtDNA from wild-type mtDNA. The proportion of mutant mtDNA is higher in ragged-red fiber (RRF) than in non-RRF in mitochondrial encephalomyopathies with mutations of mtDNA. This method may be applied to evaluate amount of...

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PMID: 9436453

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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[Single-fiber polymerase chain reaction for detection of mutant mitochondrial DNA].PMID 9436453Read ↗

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Detection and analysis of mitochondrial DNA and RNA in muscle by in situ hybridization and single-fiber PCR.Semantic proximity Read ↗

Simple detection of tRNA(Lys) mutation in myoclonus epilepsy associated with ragged-red fibers (MERRF) by polymerase chain reaction with a mismatched primer.Semantic proximity Read ↗

Detection of mitochondrial DNA (mtDNA) mutations.Semantic proximity Read ↗

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