Article
Simple detection of tRNA(Lys) mutation in myoclonus epilepsy associated with ragged-red fibers (MERRF) by polymerase chain reaction with a mismatched primer.
Neurology - 1 Nov 1991
Yoneda M, Tanno Y, Nonaka I, Miyatake T, Tsuji S
Abstract excerpt
We developed a simple method for the detection of a tRNA(Lys) mutation in myoclonus epilepsy associated with ragged-red fibers (MERRF) by polymerase chain reaction with use of a mismatched primer. Although the tRNA(Lys) mutation does not alter recognition sequences for commercially available rest...
Topics
- Base Sequence
- DNA, Mitochondrial
- Epilepsies, Myoclonic
- Female
- Humans
- Male
- Mitochondria, Muscle
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA, Transfer, Lys