Article
Study of mitochondrial DNA depletion in muscle by single-fiber polymerase chain reaction.
Muscle & nerve - 1 Nov 1998
Sciacco M, Gasparo-Rippa P, Vu T H, Tanji K, Shanske S, Mendell J R, Schon E A, DiMauro S, Bonilla E
Abstract excerpt
We studied muscle biopsies from 3 children with a mitochondrial myopathy characterized histochemically by the presence of ragged-red fibers (RRF) and various numbers of cytochrome c oxidase (COX)-negative fibers. We quantitated the absolute amounts of total mitochondrial DNA (mtDNA) in isolated normal COX-positive muscle fibers and in COX-negative RRF. There was severe mtDNA depletion in all fibers from the two...
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