Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames.

Abstract excerpt

Using a strategy developed by R. Andino, D. Silvera, S. D. Suggett, P. I. Achacoso, C. J. Miller, D. Baltimore, and M. B. Feinberg (Science 265:1448-1451, 1994), we constructed recombinant polioviruses by fusing the open reading frame (ORF) of the green fluorescent protein gene (gfp) of Aequorea...

Topics

• Amino Acid Sequence
• Base Sequence
• Cardiovirus
• DNA Primers
• Gene Expression
• Gene Products, gag
• Genetic Variation
• Genetic Vectors
• Genotype
• Green Fluorescent Proteins
• HIV-1
• HeLa Cells
• Humans
• Luminescent Proteins
• Models, Genetic
• Molecular Sequence Data
• Open Reading Frames
• Poliovirus