Article
Cloning and characterization of a novel macrolide efflux gene, mreA, from Streptococcus agalactiae.
Antimicrobial agents and chemotherapy - 1 Dec 1997
Clancy J, Dib-Hajj F, Petitpas J W, Yuan W
Abstract excerpt
A strain of Streptococcus agalactiae displayed resistance to 14-, 15-, and 16-membered macrolides. In PCR assays, total genomic DNA from this strain contained neither erm nor mef genes. EcoRI-digested genomic DNA from this strain was cloned into lambda Zap II to construct a library of S. agalactiae genomic DNA. A clone, pAES63, expressing resistance to erythromycin, azithromycin, and spiramycin in Escherichia...
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