Isolation and characterization of new alleles of the cyclin-dependent kinase gene CDC28 with cyclin-specific functional and biochemical defects.

doi:10.1128/MCB.18.1.290

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Article

Isolation and characterization of new alleles of the cyclin-dependent kinase gene CDC28 with cyclin-specific functional and biochemical defects.

Molecular and cellular biology - 1 Jan 1998

Levine K, Oehlen L J, Cross F R

Abstract excerpt

The G1 cyclin Cln2 negatively regulates the mating-factor pathway. In a genetic screen to identify factors required for this regulation, we identified an allele of CDC28 (cdc28-csr1) that blocked this function of Cln2. Cln2 immunoprecipitated from cdc28-csr1 cells was completely defective in hist...

Topics

Alleles
CDC28 Protein Kinase, S cerevisiae
Mutagenesis
Mutation
Saccharomyces cerevisiae

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