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Article

Identification and characterization of a catalytic base in bacterial luciferase by chemical rescue of a dark mutant.

Biochemistry - 2 Dec 1997

Huang S, Tu S C

Abstract excerpt

Mutation of the His44 residue of the alpha subunit of Vibrio harveyi luciferase to an alanine was known to reduce the enzyme bioluminescence activity by five orders of magnitude [Xin, X., Xi, L., and Tu, S.-C. (1991) Biochemistry 30, 11255-11262]. We found that the residual activity of the alpha H44A luciferase was markedly enhanced by exogenously added imidazole and other simple amines. The peak luminescence...

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Identifiers

PMID: 9402752
DOI: 10.1021/bi9722554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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