Article
Chemical rescue of a mutant enzyme in living cells.
Science (New York, N.Y.) - 3 Mar 2006
Qiao Yingfeng, Molina Henrik, Pandey Akhilesh, Zhang Jin, Cole Philip A
Abstract excerpt
The restoration of catalytic activity to mutant enzymes by small molecules is well established for in vitro systems. Here, we show that the protein tyrosine kinase Src arginine-388-->alanine (R388A) mutant can be rescued in live cells with the use of the small molecule imidazole. Cellular rescue of a viral Src homolog was rapid and reversible and conferred predicted oncogenic properties. Using chemical rescue in...
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