Article
Differentiation of cultured neuroblastoma induced by staurosporine and cyclic AMP: methods for assessing a neuronal phenotype.
Brain research. Brain research protocols - 1 Oct 1997
Sasaki K, Maruyama K, Nishimura E, Tsukada T, Yamaguchi K
Abstract excerpt
In cultured neuronal cells, neurite outgrowth provides a prominent morphological marker for assessing neurotrophic activity derived from different sources. Permanent cell lines are often used for such studies; however, the process of differentiation should be evaluated biochemically or electrophysiologically to determine whether the affected cells acquire a neuronal phenotype. We have reported that low-dose...
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