Article
Gene trapping with GFP: the isolation of developmental mutants in the slime mold Polysphondylium.
Current biology : CB - 1 Nov 1997
Fey P, Cox E C
Abstract excerpt
In order to study how a cell mass undergoes a transition from one symmetry to another in the slime mold Polysphondylium, we developed a genetic screen in which mutant phenotype and gene expression can easily be visualized in the living organism. The screen combines restriction enzyme-mediated integration (REMI) [1,2] and green fluorescent protein (GFP) [3] expression. In REMI, a restriction enzyme is...
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