Gene trapping with GFP: the isolation of developmental mutants in the slime mold Polysphondylium.

doi:10.1016/s0960-9822(06)00384-8

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Article

Gene trapping with GFP: the isolation of developmental mutants in the slime mold Polysphondylium.

Current biology : CB - 1 Nov 1997

Fey P, Cox E C

Abstract excerpt

In order to study how a cell mass undergoes a transition from one symmetry to another in the slime mold Polysphondylium, we developed a genetic screen in which mutant phenotype and gene expression can easily be visualized in the living organism. The screen combines restriction enzyme-mediated int...

Topics

Cloning, Molecular
Green Fluorescent Proteins
Luminescent Proteins
Mutation
Myxomycetes
Phenotype
Plasmids
Restriction Mapping
Transformation, Genetic

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