Article
Production of the homologous pectin lyase B protein in six genetically defined protease-deficient Aspergillus niger mutant strains.
Current genetics - 1 Jul 1997
van den Hombergh J P, Fraissinet-Tachet L, van de Vondervoort P J, Visser J
Abstract excerpt
An expression cassette has been transformed into six protease-deficient (prt) mutant strains of Aspergillus niger. Transformants were tested for improved production of the proteolytically susceptible PELB tester protein. In four complementation groups (prtA, B, D and F) distinct improvement of PELB yield was observed. These in vivo experiments in single prt mutants confirmed earlier in vitro PELB degradation data...
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