Article
Dynamic molecular combing: stretching the whole human genome for high-resolution studies.
Science (New York, N.Y.) - 5 Sept 1997
Michalet X, Ekong R, Fougerousse F, Rousseaux S, Schurra C, Hornigold N, van Slegtenhorst M, Wolfe J, Povey S, Beckmann J S, Bensimon A
Abstract excerpt
DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.