Article
A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2.
Genetics - 1 Jul 1997
McKee A H, Kleckner N
Abstract excerpt
We describe a general new approach for identifying recessive mutations that affect diploid strains of yeast Saccharomyces cerevisiae and the application of this method to the identification of mutations that confer an intermediate block in meiotic prophase chromosome metabolism. The method uses a...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Endonucleases
- Freezing
- Fungal Proteins
- Genes, Fungal
- Genes, Recessive
- Meiosis
- Molecular Sequence Data
- Mutation
- Phenotype
- Prophase
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins