Characterization of an HPr kinase mutant of Staphylococcus xylosus.

doi:10.1128/JB.182.7.1895-1902.2000

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Article

Characterization of an HPr kinase mutant of Staphylococcus xylosus.

Journal of bacteriology - 1 Apr 2000

Huynh P L, Jankovic I, Schnell N F, Brückner R

Abstract excerpt

The Staphylococcus xylosus gene hprK, encoding HPr kinase (HPrK), has been isolated from a genomic library. The HPrK enzyme, purified as a His(6) fusion protein, phosphorylated HPr, the phosphocarrier protein of the bacterial phosphotransferase system, at a serine residue in an ATP-dependent manner, and it also catalyzed the reverse reaction. Therefore, the enzyme constitutes a bifunctional HPr...

Topics

Adenosine Triphosphate
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Carbohydrate Metabolism
Cloning, Molecular
Gene Expression Regulation, Bacterial
Glucose
Glycoside Hydrolases
Molecular Sequence Data
Mutagenesis, Insertional

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Phenotype

Phosphoenolpyruvate Sugar Phosphotransferase System

Phosphoprotein Phosphatases

Phosphorylation

Protein Serine-Threonine Kinases

Pyruvaldehyde

Source

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Identifiers

PMID: 10714994
DOI: 10.1128/JB.182.7.1895-1902.2000
PMCID: PMC101872

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