Article
Characterization of an HPr kinase mutant of Staphylococcus xylosus.
Journal of bacteriology - 1 Apr 2000
Huynh P L, Jankovic I, Schnell N F, Brückner R
Abstract excerpt
The Staphylococcus xylosus gene hprK, encoding HPr kinase (HPrK), has been isolated from a genomic library. The HPrK enzyme, purified as a His(6) fusion protein, phosphorylated HPr, the phosphocarrier protein of the bacterial phosphotransferase system, at a serine residue in an ATP-dependent manner, and it also catalyzed the reverse reaction. Therefore, the enzyme constitutes a bifunctional HPr...
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