Article
Immortalization of caprine fibroblasts permissive for replication of small ruminant lentiviruses.
American journal of veterinary research - 1 Jun 1997
Da Silva Teixeira M F, Lambert V, Mselli-Lakahl L, Chettab A, Chebloune Y, Mornex J F
Abstract excerpt
OBJECTIVE: To establish immortalized caprine fibroblastic cell lines permissive for replication of small ruminant lentiviruses. ANIMALS: Carpal synovial membrane explants collected aseptically from a surgically delivered fetus of a lentivirus-seronegative goat. PROCEDURE: Immortalization of goat embryonic fibroblasts was performed by DNA transfection with plasmids coding for simian virus 40 large T antigen. The...
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