Article
Bone tissue-specific transcription of the osteocalcin gene: role of an activator osteoblast-specific complex and suppressor hox proteins that bind the OC box.
Journal of cellular biochemistry - 1 May 1996
Hoffmann H M, Beumer T L, Rahman S, McCabe L R, Banerjee C, Aslam F, Tiro J A, van Wijnen A J, Stein J L, Stein G S, Lian J B
Abstract excerpt
Bone-specific expression of the osteocalcin gene is transcriptionally controlled. Deletion analysis of osteocalcin promoter sequences by transient transfection of osseous (ROS 17/2.8) and nonosseous (R2 fibroblast) cells revealed that the most proximal 108 nucleotides are sufficient to confer tissue-specific expression. By gel mobility shift assays with wild-type and mutated oligonucleotides and nuclear extracts...
Topics
- Animals
- Binding Sites
- Cell Differentiation
- HeLa Cells
- Homeodomain Proteins
- Hot Temperature
- Humans
- Mutation
- Nucleotides
- Oligonucleotides, Antisense
- Osteoblasts
- Osteocalcin
- Promoter Regions, Genetic