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Article

Translation initiation factor-dependent extracts from yeast Saccharomyces cerevisiae.

Methods (San Diego, Calif.) - 1 Apr 1997

Altmann M, Trachsel H

Abstract excerpt

Translation initiation factor-dependent extracts are prepared from Saccharomyces cerevisiae strains that have no or reduced activity of a translation initiation factor. Elimination of factor activity can be achieved by deletion of the gene encoding the factor if it is not essential for the survival of the strain. If the gene is essential it is placed under the control of the regulatable GAL1 promoter and its...

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PMID: 9126549
DOI: 10.1006/meth.1996.0432

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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