Article
Induction of a type 1 immune response to a recombinant antigen from Mycobacterium tuberculosis expressed in Mycobacterium vaccae.
Infection and immunity - 1 May 1997
Abou-Zeid C, Gares M P, Inwald J, Janssen R, Zhang Y, Young D B, Hetzel C, Lamb J R, Baldwin S L, Orme I M, Yeremeev V, Nikonenko B V, Apt A S
Abstract excerpt
A 19-kDa lipoprotein from Mycobacterium tuberculosis was expressed as a recombinant antigen in the nonpathogenic mycobacterial host strain M. vaccae. Immunization of mice with the recombinant M. vaccae resulted in induction of a strong type 1 immune response to the 19-kDa antigen, characterized b...
Topics
- Administration, Oral
- Animals
- Antibodies, Bacterial
- Antigens, Bacterial
- Biological Assay
- Blotting, Western
- Disease Susceptibility
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Freund's Adjuvant
- Gene Expression Regulation, Bacterial
- Genetic Predisposition to Disease