Defective cation-coupling mutants of Escherichia coli Na+/proline symport carrier. Characterization and localization of mutations.

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Article

Defective cation-coupling mutants of Escherichia coli Na+/proline symport carrier. Characterization and localization of mutations.

The Journal of biological chemistry - 15 Feb 1990

Yamato I, Ohsawa M, Anraku Y

Abstract excerpt

A major proline carrier in Escherichia coli encoded by the putP gene mediates proline/Na+ or Li+ symport. Proline carrier mutants with altered cation specificity were obtained by mutagenesis with nitrous acid in vitro of a plasmid carrying the wild-type putP gene. Two mutant strains harboring plasmid pMOP4135 and pMOP4141 could transport proline efficiently only in the presence of an increased concentration of...

Topics

Amino Acid Sequence
Amino Acid Transport Systems, Neutral
Base Sequence
Biological Transport
Carrier Proteins
Cell Membrane
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Genotype
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Oligonucleotide Probes

Plasmids

Proline

Sodium

Symporters

Source

Open in PubMed

Identifiers

PMID: 2406235

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2406235-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Defective cation-coupling mutants of Escherichia coli Na+/proline symport carrier. Characterization and localization of mutations.PMID 2406235Read ↗

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